Journal: International Journal of Clinical and Experimental Pathology

Article Title: Correlation of Wnt antagonist sFPR1, Slug and β-catenin with prognosis and metastasis in colorectal carcinoma

doi:

Figure Lengend Snippet: Expression of the proteins in colorectal carcinoma (×400 magnification). A. Positive sFPR1 expression in the cytoplasm of “normal” mucosa cells. B. Positive sFPR1 expression in the cytoplasm of cancer cells. C. Positive Slug expression in the cytoplasm of “normal” mucosa cells. D. Positive Slug expression in the cytoplasm of cancer cells. E. Positive β-catenin expression in the membrane of “normal” mucosacells. F. Positive β-catenin expression in the membrane of cancer cells. G. Positive β-catenin expression in the nucleus of cancer cells. H. Positive β-catenin expression in the nucleus and cytoplasm of cancer cells.

Article Snippet: The membrane was blocked using 5% skimmed milk for 1 h. Primary antibody sFPR1 (1:1000), Slug (1:1000), and β-catenin (1:1000) were added at 4°C overnight, followed by horseradish peroxidase-labeled rabbit anti-goat secondary antibody (1:10000), and shacked for 2 h. The Bio-Rad imaging system was used for imaging and acquisition.

Techniques: Expressing, Membrane

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Correlation of Wnt antagonist sFPR1, Slug and β-catenin with prognosis and metastasis in colorectal carcinoma

doi:

Figure Lengend Snippet: The relationship between expression of sFPR1, β-catenin, Slug and clinicopathogical characteristics of (CRC)

Article Snippet: The membrane was blocked using 5% skimmed milk for 1 h. Primary antibody sFPR1 (1:1000), Slug (1:1000), and β-catenin (1:1000) were added at 4°C overnight, followed by horseradish peroxidase-labeled rabbit anti-goat secondary antibody (1:10000), and shacked for 2 h. The Bio-Rad imaging system was used for imaging and acquisition.

Techniques: Expressing, Membrane

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Correlation of Wnt antagonist sFPR1, Slug and β-catenin with prognosis and metastasis in colorectal carcinoma

doi:

Figure Lengend Snippet: Correlation between expression of sFPR1, Slug, β-catenin in CRC

Article Snippet: The membrane was blocked using 5% skimmed milk for 1 h. Primary antibody sFPR1 (1:1000), Slug (1:1000), and β-catenin (1:1000) were added at 4°C overnight, followed by horseradish peroxidase-labeled rabbit anti-goat secondary antibody (1:10000), and shacked for 2 h. The Bio-Rad imaging system was used for imaging and acquisition.

Techniques: Expressing

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Correlation of Wnt antagonist sFPR1, Slug and β-catenin with prognosis and metastasis in colorectal carcinoma

doi:

Figure Lengend Snippet: Kaplan-Meier analysis of the survival rate of patients with colorectal carcinoma. (A) Overall survival of all patients in relation to sFPR1 expression (log-rank = 17.415, P < 0.001). (B) Overall survival of all patients in relation to β-catenin expression (log-rank = 21.387, P < 0.001). (C) Overall survival of all patients in relation to Slug expression (log-rank = 10.415, P = 0.001). In (A-C) analyses, the green line represents positive expression of proteins and the blue line represents negative expression of proteins. (D) Overall survival of all patients in relation to the combination of sFPR1, β-catenin and Slug expression (log-rank = 34.157, P < 0.001). The green line represents positive expression of sFRP1 and negative expression of Slug, β-catenin and the blue line represents negative expression of sFRP1 and positive expression of Slug, β-catenin. The red line represents other positive or negative expression of the proteins. In all analyses, †represents censored observation.

Article Snippet: The membrane was blocked using 5% skimmed milk for 1 h. Primary antibody sFPR1 (1:1000), Slug (1:1000), and β-catenin (1:1000) were added at 4°C overnight, followed by horseradish peroxidase-labeled rabbit anti-goat secondary antibody (1:10000), and shacked for 2 h. The Bio-Rad imaging system was used for imaging and acquisition.

Techniques: Expressing

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Correlation of Wnt antagonist sFPR1, Slug and β-catenin with prognosis and metastasis in colorectal carcinoma

doi:

Figure Lengend Snippet: Results of univariate analyses of (OS) time

Article Snippet: The membrane was blocked using 5% skimmed milk for 1 h. Primary antibody sFPR1 (1:1000), Slug (1:1000), and β-catenin (1:1000) were added at 4°C overnight, followed by horseradish peroxidase-labeled rabbit anti-goat secondary antibody (1:10000), and shacked for 2 h. The Bio-Rad imaging system was used for imaging and acquisition.

Techniques: Expressing, Membrane

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Correlation of Wnt antagonist sFPR1, Slug and β-catenin with prognosis and metastasis in colorectal carcinoma

doi:

Figure Lengend Snippet: Results of multivariate analyses of (OS) time

Article Snippet: The membrane was blocked using 5% skimmed milk for 1 h. Primary antibody sFPR1 (1:1000), Slug (1:1000), and β-catenin (1:1000) were added at 4°C overnight, followed by horseradish peroxidase-labeled rabbit anti-goat secondary antibody (1:10000), and shacked for 2 h. The Bio-Rad imaging system was used for imaging and acquisition.

Techniques:

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Correlation of Wnt antagonist sFPR1, Slug and β-catenin with prognosis and metastasis in colorectal carcinoma

doi:

Figure Lengend Snippet: The expression of sFPR1, β-catenin and Slug was determined by Western blotting in the SW480 cell line treated with 5-aza-dc.

Article Snippet: The membrane was blocked using 5% skimmed milk for 1 h. Primary antibody sFPR1 (1:1000), Slug (1:1000), and β-catenin (1:1000) were added at 4°C overnight, followed by horseradish peroxidase-labeled rabbit anti-goat secondary antibody (1:10000), and shacked for 2 h. The Bio-Rad imaging system was used for imaging and acquisition.

Techniques: Expressing, Western Blot